mouse TARBP2 shRNA silencing Adenovirus

Name
Ad-m-TARBP2-shRNA

Cat No
shADV-273696

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Tarbp2.

shADV-273696

Ad-m-TARBP2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Tarbp2 (TARBP2, RISC loading complex RNA binding subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

Prbp; TRBP

Description (Vector)

Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.

Gene ID

21357

Gene Name (Vector)

TARBP2, RISC loading complex RNA binding subunit

Gene Symbol

Tarbp2

ORF Size (aa)

1098

ORF Size (bp)

1098 bp

RefSeq ID

NM_009319

RefSeq Synonyms

NM_009319, NM_001253795, BC002028,

Species

mouse

UniGene ID

Mm.291485

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.