mouse SPC25 shRNA silencing Adenovirus

Name 
Ad-m-SPC25-shRNA
Cat No 
shADV-272967
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Spc25.

shADV-272967
Ad-m-SPC25-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Spc25 (SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
2600017H08Rik; 2610205L13Rik; Spbc25
Description (Vector)
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
Gene ID
66442
Gene Name (Vector)
SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Gene Symbol
Spc25
ORF Size (aa)
681
ORF Size (bp)
681 bp
RefSeq ID
NM_025565
RefSeq Synonyms
NM_025565, NM_001305800, NM_001199124, NM_001199123, BC033605, BC027121,
Species
mouse
UniGene ID
Mm.272969

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.