mouse SF3B3 shRNA silencing Adenovirus

Name 
Ad-m-SF3B3-shRNA
Cat No 
shADV-271783
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Sf3b3.

shADV-271783
Ad-m-SF3B3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Sf3b3 (splicing factor 3b, subunit 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
1810061H24Rik; 5730409A01Rik; AA409318; D8Ertd633e; mKIAA0017; RSE1; SAP130
Description (Vector)
Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Gene ID
101943
Gene Name (Vector)
splicing factor 3b, subunit 3
Gene Symbol
Sf3b3
ORF Size (aa)
3654
ORF Size (bp)
3654 bp
RefSeq ID
NM_133953
RefSeq Synonyms
NM_133953, BC139016, BC139015, BC042580, BC011412,
Species
mouse
UniGene ID
Mm.236123

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.