mouse RSAD2 shRNA silencing Adenovirus

Name 
Ad-m-RSAD2-shRNA
Cat No 
shADV-271168
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Rsad2.

shADV-271168
Ad-m-RSAD2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Rsad2 (radical S-adenosyl methionine domain containing 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
2510004L01Rik; cig5; Vig1
Description (Vector)
Involved in antiviral defense. May impair virus budding by disrupting lipid rafts at the plasma membrane, a feature which is essential for the budding process of many viruses. Acts through binding with and inactivating FPPS, an enzyme involved in synthesis of cholesterol, farnesylated and geranylated proteins, ubiquinones dolichol and heme. Plays a major role in the cell antiviral state induced by type I and type II interferon. Displays antiviral effect against HIV-1 virus, hepatitis C virus, human cytomegalovirus, and aphaviruses, but not vesiculovirus.
Gene ID
58185
Gene Name (Vector)
radical S-adenosyl methionine domain containing 2
Gene Symbol
Rsad2
ORF Size (aa)
1089
ORF Size (bp)
1089 bp
RefSeq ID
NM_021384
RefSeq Synonyms
NM_021384, BC057868,
Species
mouse
UniGene ID
Mm.24045

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.