mouse PUF60 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Puf60.
shADV-269852
Ad-m-PUF60-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Puf60 (poly-U binding splicing factor 60). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
2410104I19Rik; 2810454F19Rik; SIAHBP1
Description (Vector)
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
Gene ID
67959
Gene Name (Vector)
poly-U binding splicing factor 60
Gene Symbol
Puf60
ORF Size (aa)
1500
ORF Size (bp)
1500 bp
RefSeq ID
NM_133691
RefSeq Synonyms
NM_133691, NM_028364, NM_001164600, BC010601,
Species
mouse
UniGene ID
Mm.29965
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.