mouse PMS2 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Pms2.
shADV-268942
Ad-m-PMS2-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Pms2 (postmeiotic segregation increased 2 (S. cerevisiae)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AW555130; Pmsl2
Description (Vector)
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MulL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.
Gene ID
18861
Gene Name (Vector)
postmeiotic segregation increased 2 (S. cerevisiae)
Gene Symbol
Pms2
ORF Size (aa)
2580
ORF Size (bp)
2580 bp
RefSeq ID
NM_008886
RefSeq Synonyms
NM_008886, BC141287,
Species
mouse
UniGene ID
Mm.2950
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.