mouse MPRIP shRNA silencing Adenovirus

Name 
Ad-m-MPRIP-shRNA
Cat No 
shADV-264852
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Mprip.

shADV-264852
Ad-m-MPRIP-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Mprip (myosin phosphatase Rho interacting protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
9530046C02; AA536749; AI647711; C76423; mKIAA0864; p116Rip; Rhoip3; RIP3
Description (Vector)
Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
Gene ID
26936
Gene Name (Vector)
myosin phosphatase Rho interacting protein
Gene Symbol
Mprip
ORF Size (aa)
3114
ORF Size (bp)
3114 bp
RefSeq ID
NM_201245
RefSeq Synonyms
NM_201245, NM_012027, BC063067,
Species
mouse
UniGene ID
Mm.392074

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.