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mouse MED23 shRNA silencing Adenovirus

Name 
Ad-m-MED23-shRNA
Cat No 
shADV-264450
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Med23.

shADV-264450
Ad-m-MED23-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Med23 (mediator complex subunit 23). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
130kDa; 3000002A17Rik; Crsp3; ESTM7; mKIAA1216; Sur2; X83317
Description (Vector)
Required for transcriptional activation subsequent to the assembly of the preinitiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.
Gene ID
70208
Gene Name (Vector)
mediator complex subunit 23
Gene Symbol
Med23
ORF Size (aa)
4104
ORF Size (bp)
4104 bp
RefSeq ID
NM_027347
RefSeq Synonyms
NM_027347, NM_001166416, BC050916,
Species
mouse
UniGene ID
Mm.28020

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.