mouse MED22 shRNA silencing Adenovirus

Name 
Ad-m-MED22-shRNA
Cat No 
shADV-264449
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Med22.

shADV-264449
Ad-m-MED22-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Med22 (mediator complex subunit 22). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AW212655; AW558812; Surf-5; Surf5
Description (Vector)
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Gene ID
20933
Gene Name (Vector)
mediator complex subunit 22
Gene Symbol
Med22
ORF Size (aa)
423
RefSeq ID
NM_011513
RefSeq Synonyms
NM_011513, NM_001033908, BC018225,
Species
mouse
UniGene ID
Mm.397932

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.