mouse MED20 shRNA silencing Adenovirus

Name
Ad-m-MED20-shRNA

Cat No
shADV-264447

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Med20.

shADV-264447

Ad-m-MED20-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Med20 (mediator complex subunit 20). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

1110011O05Rik; 2410115I17Rik; AU018348; Trfp

Description (Vector)

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene ID

56771

Gene Name (Vector)

mediator complex subunit 20

Gene Symbol

Med20

ORF Size (aa)

438

ORF Size (bp)

438 bp

RefSeq ID

NM_020048

RefSeq Synonyms

NM_020048, BC060122,

Species

mouse

UniGene ID

Mm.440560

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.