mouse MED10 shRNA silencing Adenovirus

Name
Ad-m-MED10-shRNA

Cat No
shADV-264435

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Med10.

shADV-264435

Ad-m-MED10-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Med10 (mediator complex subunit 10). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

AA959813; AI385605; C78613; D13Wsu50e

Description (Vector)

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Gene ID

28077

Gene Name (Vector)

mediator complex subunit 10

Gene Symbol

Med10

ORF Size (aa)

408

RefSeq ID

NM_138596

RefSeq Synonyms

NM_138596, BC056438, BC048638,

Species

mouse

UniGene ID

Mm.477719

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.