mouse LGTN shRNA silencing Adenovirus

Name
Ad-m-LGTN-shRNA

Cat No
shADV-263420

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Eif2d.

shADV-263420

Ad-m-LGTN-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Eif2d (eukaryotic translation initiation factor 2D). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

D1Ertd5e; Lgtn

Description (Vector)

Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P-site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits.

Gene ID

16865

Gene Name (Vector)

eukaryotic translation initiation factor 2D

Gene Symbol

Eif2d

ORF Size (aa)

1713

ORF Size (bp)

1713 bp

RefSeq ID

NM_010709

RefSeq Synonyms

NM_010709, NM_001136070, BC025036,

Species

mouse

UniGene ID

Mm.276294

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.