mouse JHDM1D shRNA silencing Adenovirus

Name 
Ad-m-JHDM1D-shRNA
Cat No 
shADV-262519
Availability
Immediate
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Kdm7a.

shADV-262519
Ad-m-JHDM1D-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Kdm7a (lysine (K)-specific demethylase 7A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
86
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
A630082K20Rik; BB041802; ENSMUSG00000073143; Jhdm1d; mKIAA1718
Description (Vector)
Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate.
Gene ID
338523
Gene Name (Vector)
lysine (K)-specific demethylase 7A
Gene Symbol
Kdm7a
ORF Size (aa)
2670
ORF Size (bp)
2670 bp
RefSeq ID
NM_001033430
RefSeq Synonyms
NM_001033430, BC145848,
Species
mouse
UniGene ID
Mm.293175

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.