mouse HDAC1 shRNA silencing Adenovirus

Name 
Ad-m-HDAC1-shRNA
Cat No 
shADV-261170
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Hdac1.

shADV-261170
Ad-m-HDAC1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Hdac1 (histone deacetylase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
HD1; Hdac1-ps; MommeD5; RPD3
Description (Vector)
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Component a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
Gene ID
433759
Gene Name (Vector)
histone deacetylase 1
Gene Symbol
Hdac1
ORF Size (aa)
1449
ORF Size (bp)
1449 bp
RefSeq ID
NM_008228
RefSeq Synonyms
NM_008228, BC108371, BC092070,
Species
mouse
UniGene ID
Mm.391033

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.