mouse EZH2 shRNA silencing Adenovirus

Name 
Ad-m-EZH2-shRNA
Cat No 
shADV-258824
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Ezh2.

shADV-258824
Ad-m-EZH2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Ezh2 (enhancer of zeste 2 polycomb repressive complex 2 subunit). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Enx-1; Enx1h; KMT6; mKIAA4065
Description (Vector)
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Gene ID
14056
Gene Name (Vector)
enhancer of zeste 2 polycomb repressive complex 2 subunit
Gene Symbol
Ezh2
ORF Size (aa)
2229
ORF Size (bp)
2229 bp
RefSeq ID
NM_007971
RefSeq Synonyms
NM_007971, NM_001146689, BC079538, BC016391, BC003772,
Species
mouse
UniGene ID
Mm.246688

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.