mouse ENTPD5 shRNA silencing Adenovirus

Name 
Ad-m-ENTPD5-shRNA
Cat No 
shADV-258532
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Entpd5.

shADV-258532
Ad-m-ENTPD5-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Entpd5 (ectonucleoside triphosphate diphosphohydrolase 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AI196558; AI987697; Cd39l4; ER-UDPase; mNTPase; NTPDase-5; NTPDase5; Pcph
Description (Vector)
Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. Also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling (By similarity).
Gene ID
12499
Gene Name (Vector)
ectonucleoside triphosphate diphosphohydrolase 5
Gene Symbol
Entpd5
ORF Size (aa)
1284
RefSeq ID
NM_007647
RefSeq Synonyms
NM_007647, NM_001286058, NM_001286049, NM_001026214, BC015247,
Species
mouse
UniGene ID
Mm.10211

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.