mouse PIK3C2A shRNA silencing Adenovirus

Name
Ad-m-PIK3C2A-shRNA

Cat No
shADV-258512

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Pik3c2a.

shADV-258512

Ad-m-PIK3C2A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Pik3c2a (phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

Cpk-m; PI3KC2

Description (Vector)

Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function.

Gene ID

18704

Gene Name (Vector)

phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide

Gene Symbol

Pik3c2a

ORF Size (aa)

5058

ORF Size (bp)

5058 bp

RefSeq ID

NM_011083

RefSeq Synonyms

NM_011083, BC156426,

Species

mouse

UniGene ID

Mm.3810

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.