mouse EEF2K shRNA silencing Adenovirus

Name 
Ad-m-EEF2K-shRNA
Cat No 
shADV-257788
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Eef2k.

shADV-257788
Ad-m-EEF2K-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Eef2k (eukaryotic elongation factor-2 kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
C86191; eEF-2K
Description (Vector)
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
Gene ID
13631
Gene Name (Vector)
eukaryotic elongation factor-2 kinase
Gene Symbol
Eef2k
ORF Size (aa)
2175
ORF Size (bp)
2175 bp
RefSeq ID
NM_007908
RefSeq Synonyms
NM_007908, NM_001267711, NM_001267710, BC055361, BC003433,
Species
mouse
UniGene ID
Mm.361795

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.