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mouse BUB1B shRNA silencing Adenovirus

Name 
Ad-m-BUB1B-shRNA
Cat No 
shADV-254170
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Bub1b.

shADV-254170
Ad-m-BUB1B-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Bub1b (BUB1B, mitotic checkpoint serine/threonine kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AU045529; BUBR1
Description (Vector)
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Gene ID
12236
Gene Name (Vector)
BUB1B, mitotic checkpoint serine/threonine kinase
Gene Symbol
Bub1b
ORF Size (aa)
3159
ORF Size (bp)
3159 bp
RefSeq ID
NM_009773
RefSeq Synonyms
NM_009773, BC031577,
Species
mouse
UniGene ID
Mm.29133

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.