mouse BBS9 shRNA silencing Adenovirus

Name
Ad-m-BBS9-shRNA

Cat No
shADV-253664

Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Bbs9.

shADV-253664

Ad-m-BBS9-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Bbs9 (Bardet-Biedl syndrome 9 (human)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

E130103I17Rik

Description (Vector)

The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane.

Gene ID

319845

Gene Name (Vector)

Bardet-Biedl syndrome 9 (human)

Gene Symbol

Bbs9

ORF Size (aa)

2643

ORF Size (bp)

2643 bp

RefSeq ID

NM_181316

RefSeq Synonyms

NM_181316, NM_178415, BC046426,

Species

mouse

UniGene ID

Mm.176725

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.