mouse ATPBD3 shRNA silencing Adenovirus

Name 
Ad-m-ATPBD3-shRNA
Cat No 
shADV-253371
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Mouse Ctu1.

shADV-253371
Ad-m-ATPBD3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Ctu1 (cytosolic thiouridylase subunit 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
Atpbd3; BC005752
Description (Vector)
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
Gene ID
233189
Gene Name (Vector)
cytosolic thiouridylase subunit 1
Gene Symbol
Ctu1
ORF Size (aa)
1263
ORF Size (bp)
1263 bp
RefSeq ID
NM_145582
RefSeq Synonyms
NM_145582, BC005752,
Species
mouse
UniGene ID
Mm.26514

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.