mouse AOF1 shRNA silencing Adenovirus

Name 
Ad-m-AOF1-shRNA
Cat No 
shADV-252754
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Mouse Kdm1b.

shADV-252754
Ad-m-AOF1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Kdm1b (lysine (K)-specific demethylase 1B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
4632428N09Rik; AI482520; Aof1
Description (Vector)
Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity).
Gene ID
218214
Gene Name (Vector)
lysine (K)-specific demethylase 1B
Gene Symbol
Kdm1b
ORF Size (aa)
2481
ORF Size (bp)
2481 bp
RefSeq ID
NM_172262
RefSeq Synonyms
NM_172262, BC023917,
Species
mouse
UniGene ID
Mm.31259

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.