mouse AMFR shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Amfr.
shADV-252563
Ad-m-AMFR-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Amfr (autocrine motility factor receptor). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
gp78
Description (Vector)
Specific receptor for the autocrine motility factor|Function: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis.
Gene ID
23802
Gene Name (Vector)
autocrine motility factor receptor
Gene Symbol
Amfr
ORF Size (aa)
1920
ORF Size (bp)
1920 bp
RefSeq ID
NM_011787
RefSeq Synonyms
NM_011787, BC040338, BC034538,
Species
mouse
UniGene ID
Mm.34641
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.