mouse AKT1S1 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Mouse Akt1s1.
shADV-252434
Ad-m-AKT1S1-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Mouse Akt1s1 (AKT1 substrate 1 (proline-rich)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
1110012J22Rik; AI227026; Lobe; Lobel; PRAS40
Description (Vector)
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection.
Gene ID
67605
Gene Name (Vector)
AKT1 substrate 1 (proline-rich)
Gene Symbol
Akt1s1
ORF Size (aa)
774
RefSeq ID
NM_026270
RefSeq Synonyms
NM_026270, NM_001290695, NM_001290694, NM_001253920, BC132374, BC132372,
Species
mouse
UniGene ID
Mm.431538
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.