human ATP6V0E2 shRNA silencing Adenovirus

Name
Ad-h-ATP6V0E2-shRNA

Cat No
shADV-234179

Availability
5-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human ATP6V0E2.

shADV-234179

Ad-h-ATP6V0E2-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human ATP6V0E2 (ATPase H+ transporting V0 subunit e2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

shRNA Validated

Yes

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

ATP6V0E2L; C7orf32

Description (Vector)

Multisubunit vacuolar-type proton pumps, or H(+)-ATPases, acidify various intracellular compartments, such as vacuoles, clathrin-coated and synaptic vesicles, endosomes, lysosomes, and chromaffin granules. H(+)-ATPases are also found in plasma membranes of specialized cells, where they play roles in urinary acidification, bone resorption, and sperm maturation. Multiple subunits form H(+)-ATPases, with proteins of the V1 class hydrolyzing ATP for energy to transport H+, and proteins of the V0 class forming an integral membrane domain through which H+ is transported. ATP6V0E2 encodes an isoform of the H(+)-ATPase V0 e subunit, an essential proton pump component (Blake-Palmer et al., 2007 (PubMed 17350184)).(supplied by OMIM, Mar 2008)

Gene ID

155066

Gene Name (Vector)

ATPase H+ transporting V0 subunit e2

Gene Symbol

ATP6V0E2

HGNC ID

HGNC:21723

ORF Size (aa)

642

RefSeq ID

NM_145230

RefSeq Synonyms

NM_145230, NM_001289990, NM_001100592, BC015899,

Species

human

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.