human PRKCSH shRNA silencing Adenovirus

Name 
Ad-h-PRKCSH-shRNA
Cat No 
shADV-233920
Availability
5-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human PRKCSH.

shADV-233920
Ad-h-PRKCSH-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PRKCSH (protein kinase C substrate 80K-H). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
80K-H protein; advanced glycation end-product receptor 2; AGE-binding receptor 2; AGE-R2; G19P1; GIIB; glucosidase 2 subunit beta; glucosidase II subunit beta; hepatocystin; PCLD; PCLD1; PKCSH; PLD1; protein kinase C substrate 60.1 kDa protein heavy chain; protein kinase C substrate, 80 Kda protein; VASAP-60
Description (eCommerce)
Protein kinase C substrate 60.1 kDa protein heavy chain (PRKCSH) is a regulatory subunit of glucosidase II [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14314].
Description (Vector)
PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease (PCLD). Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene ID
5589
Gene Name (eCommerce)
protein kinase C substrate 80K-H
Gene Name (Vector)
protein kinase C substrate 80K-H
Gene Symbol
PRKCSH
HGNC ID
HGNC:9411
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1521
ORF Size (bp)
1521 bp
Protein Name (eCommerce)
Glucosidase 2 subunit beta
RefSeq ID
NM_001001329
RefSeq Synonyms
NM_002743, NM_001289104, NM_001289103, NM_001289102, NM_001001329, BC013586,
Research Areas
Immunology,Innate Immunity,Metabolism/Metabolic Process,Protein Folding,RNA Binding,Signal Transduction,Translational Control
Research Areas (Faceted)
cell_biology,immunology,metabolism,signal_transduction,transcription_translation
Species
human
Target Sentence
Protein kinase C substrate 60.1 kDa protein heavy chain (PRKCSH) is a regulatory subunit of glucosidase II [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14314].
UniProt ID (eCommerce)
P14314

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.