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human SMG1 shRNA silencing Adenovirus

Name 
Ad-h-SMG1-shRNA
Cat No 
shADV-231205
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SMG1.

shADV-231205
Ad-h-SMG1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SMG1 (SMG1, nonsense mediated mRNA decay associated PI3K related kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
61E3.4; ATX; hSMG-1; lambda/iota protein kinase C-interacting protein; lambda-interacting protein; LIP; PI-3-kinase-related kinase SMG-1; serine/threonine-protein kinase SMG1; SMG-1; smg-1 homolog, phosphatidylinositol 3-kinase-related kinase; SMG1 phosphatidylinositol 3-kinase-related kinase
Description (eCommerce)
SMG1 is a PIKK (phosphoinositide 3-kinase related kinases) protein kinase that plays a key role in RNA surveillance via nonsense-mediated mRNA decay (NMD). SMG1 phosphorylates Upf1, a factor that possesses RNA helicase activity and is also central to NMD. SMG1 and UPF-1 have been shown to exist in a complex with the translation termination release factors eRF3, and eRF1. This complex has been termed SURF and associates with the exon junction complex (EJC) to function in the recognition of premature termination codons in aberrant RNAs to trigger NMD.
Description (Vector)
SMG1 encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternative spliced transcript variants have been described, but their full-length natures have not been determined.
Gene ID
23049
Gene Name (eCommerce)
SMG1, nonsense mediated mRNA decay associated PI3K related kinase
Gene Name (Vector)
SMG1, nonsense mediated mRNA decay associated PI3K related kinase
Gene Symbol
SMG1
HGNC ID
HGNC:30045
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
10983
ORF Size (bp)
10983 bp
Protein Name (eCommerce)
Serine/threonine-protein kinase SMG1
RefSeq ID
NM_015092
RefSeq Synonyms
NM_015092, BC156497,
Research Areas
DNA Damage/Repair,Gene Expression,Kinase/Phosphatase,Nuclear Import/Export,RNA Processing,Transport
Research Areas (Faceted)
cell_biology,genetics,signal_transduction,transcription_translation
Species
human
Target Sentence
SMG1 is a PIKK (phosphoinositide 3-kinase related kinases) protein kinase that plays a key role in RNA surveillance via nonsense-mediated mRNA decay (NMD). SMG1 phosphorylates Upf1, a factor that possesses RNA helicase activity and is also central to NMD. SMG1 and UPF-1 have been shown to exist in a complex with the translation termination release factors eRF3, and eRF1. This complex has been termed SURF and associates with the exon junction complex (EJC) to function in the recognition of premature termination codons in aberrant RNAs to trigger NMD.
UniGene ID
Hs.460179
UniProt ID (eCommerce)
Q96Q15

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.