human DDX18 shRNA silencing Adenovirus

Name 
Ad-h-DDX18-shRNA
Cat No 
shADV-230166
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human DDX18.

shADV-230166
Ad-h-DDX18-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human DDX18 (DEAD-box helicase 18). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ATP-dependent RNA helicase DDX18; DEAD (Asp-Glu-Ala-Asp) box polypeptide 18; DEAD box protein 18; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 18 (Myc-regulated); Has1; MrDb; Myc-regulated DEAD box protein
Description (eCommerce)
DDX18 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX18 has been observed to be a target of myc-max heterodimers.
Description (Vector)
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein.
Gene ID
8886
Gene Name (eCommerce)
DEAD-box helicase 18
Gene Name (Vector)
DEAD-box helicase 18
Gene Symbol
DDX18
HGNC ID
HGNC:2741
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2013
ORF Size (bp)
2013 bp
Protein Name (eCommerce)
ATP-dependent RNA helicase DDX18
RefSeq ID
NM_006773
RefSeq Synonyms
NM_006773, BC024739, BC003360, BC001238,
Research Areas
Helicase Activity,RNA Binding,RNA Processing
Research Areas (Faceted)
cell_biology,genetics,transcription_translation
Species
human
Target Sentence
DDX18 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. DDX18 has been observed to be a target of myc-max heterodimers.
UniGene ID
Hs.363492
UniProt ID (eCommerce)
Q9NVP1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.