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human WNK3 shRNA silencing Adenovirus

Name 
Ad-h-WNK3-shRNA
Cat No 
shADV-227808
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human WNK3.

shADV-227808
Ad-h-WNK3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human WNK3 (WNK lysine deficient protein kinase 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
PRKWNK3; Protein kinase lysine-deficient 3; protein kinase with no lysine 3; serine/threonine-protein kinase WNK3
Description (eCommerce)
WNK3 (With No Lysine 3) is a member of the WNK family of serine-threonine kinases which includes WNK1, WNK2, WNK3, and WNK4. The WNK family of kinases has been observed to play a role in several cellular processes which include ion balance, signal transduction, survival, proliferation and organ development. WNK3 appears to play a role in NaCl, water, and blood pressure homeostasis but has properties distinct from the other WNK family members. WNK3 is expressed throughout the nephron, particularly at intercellular junctions, and its kinase activity has been shown to regulate the activity of ion transporters such as the Na-K-2Cl and Na-Cl co-transporters.
Description (Vector)
WNK3 encodes a protein belonging to the 'with no lysine' family of serine-threonine protein kinases. These family members lack the catalytic lysine in subdomain II, and instead have a conserved lysine in subdomain I. This family member functions as a positive regulator of the transcellular Ca2+ transport pathway, and it plays a role in the increase of cell survival in a caspase-3-dependent pathway. Alternative splicing results in multiple transcript variants.
Gene ID
65267
Gene Name (eCommerce)
WNK lysine deficient protein kinase 3
Gene Name (Vector)
WNK lysine deficient protein kinase 3
Gene Symbol
WNK3
HGNC ID
HGNC:14543
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5400
ORF Size (bp)
5400 bp
Protein Name (eCommerce)
Serine/threonine-protein kinase WNK3
RefSeq ID
NM_020922
RefSeq Synonyms
NM_020922, NM_001002838, BC156469,
Research Areas
Apoptosis,Kinase/Phosphatase,Neurobiology,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology,neurobiology
Species
human
Target Sentence
WNK3 (With No Lysine 3) is a member of the WNK family of serine-threonine kinases which includes WNK1, WNK2, WNK3, and WNK4. The WNK family of kinases has been observed to play a role in several cellular processes which include ion balance, signal transduction, survival, proliferation and organ development. WNK3 appears to play a role in NaCl, water, and blood pressure homeostasis but has properties distinct from the other WNK family members. WNK3 is expressed throughout the nephron, particularly at intercellular junctions, and its kinase activity has been shown to regulate the activity of ion transporters such as the Na-K-2Cl and Na-Cl co-transporters.
UniGene ID
Hs.92423
UniProt ID (eCommerce)
Q9BYP7

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.