human WISP1 shRNA silencing Adenovirus
This is an Adenovirus expressing shRNA for silencing of Human WISP1.
shADV-227797
Ad-h-WISP1-shRNA
Ready-to-use Adenovirus expressing shRNA for silencing of Human WISP1 (WNT1 inducible signaling pathway protein 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CCN4; WISP1c; WISP1i; WISP1tc
Description (Vector)
WISP1 encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. WISP1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.
Gene ID
8840
Gene Name (Vector)
WNT1 inducible signaling pathway protein 1
Gene Symbol
WISP1
HGNC ID
HGNC:12769
ORF Size (aa)
1104
RefSeq ID
NM_003882
RefSeq Synonyms
NM_080838, NM_003882, NM_001204870, NM_001204869, BC074841, BC074840,
Species
human
UniGene ID
Hs.492974
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.