human WDR77 shRNA silencing Adenovirus

Name 
Ad-h-WDR77-shRNA
Cat No 
shADV-227728
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human WDR77.

shADV-227728
Ad-h-WDR77-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human WDR77 (WD repeat domain 77). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
androgen receptor cofactor p44; HKMT1069; MEP50; MEP-50; methylosome protein 50; Nbla10071; p44; p44/Mep50; testis tissue sperm-binding protein Li 44a; WD repeat-containing protein 77
Description (eCommerce)
WDR77/MEP50 is a component of the methylosome, a large protein arginine methyltransferase complex that modifies specific arginines to dimethylarginines in splicesomal Sm proteins. WDR77/MEP50 associates with the methyltransferase JBP1 (PRMT5) and is important for methylosome activity. WDR77/MEP50 has also been shown to associate with the polycomb group protein, SUZ12, and histone H2A. In this context, WDR77/MEP50 is proposed to function as an adaptor between PRMT5 and its substrate H2A to regulate transcription.
Description (Vector)
WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 (PubMed 11756452)).(supplied by OMIM, Mar 2008)
Gene ID
79084
Gene Name (eCommerce)
WD repeat domain 77
Gene Name (Vector)
WD repeat domain 77
Gene Symbol
WDR77
HGNC ID
HGNC:29652
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1029
Protein Name (eCommerce)
Methylosome protein 50
RefSeq ID
NM_024102
RefSeq Synonyms
NM_024102, NM_001317064, NM_001317063, NM_001317062, BC016946, BC011778, BC009411, BC006477, BC001679,
Research Areas
Gene Expression,Golgi Apparatus,Histone Modification,RNA Processing,Spliceosome,Transcription Factor/Regulator
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
WDR77/MEP50 is a component of the methylosome, a large protein arginine methyltransferase complex that modifies specific arginines to dimethylarginines in splicesomal Sm proteins. WDR77/MEP50 associates with the methyltransferase JBP1 (PRMT5) and is important for methylosome activity. WDR77/MEP50 has also been shown to associate with the polycomb group protein, SUZ12, and histone H2A. In this context, WDR77/MEP50 is proposed to function as an adaptor between PRMT5 and its substrate H2A to regulate transcription.
UniGene ID
Hs.204773
UniProt ID (eCommerce)
Q9BQA1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.