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human UBE2M shRNA silencing Adenovirus

Name 
Ad-h-UBE2M-shRNA
Cat No 
shADV-227024
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human UBE2M.

shADV-227024
Ad-h-UBE2M-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human UBE2M (ubiquitin conjugating enzyme E2 M). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
hUbc12; UBC-RS2; UBC12
Description (Vector)
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
Gene ID
9040
Gene Name (Vector)
ubiquitin conjugating enzyme E2 M
Gene Symbol
UBE2M
HGNC ID
HGNC:12491
ORF Size (aa)
552
RefSeq ID
NM_003969
RefSeq Synonyms
NM_003969, BC058924,
Species
human
UniGene ID
Hs.406068

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.