human UBE2I shRNA silencing Adenovirus

Name 
Ad-h-UBE2I-shRNA
Cat No 
shADV-227013
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human UBE2I.

shADV-227013
Ad-h-UBE2I-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human UBE2I (ubiquitin conjugating enzyme E2 I). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
C358B7.1; P18; UBC9
Description (Vector)
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene ID
7329
Gene Name (Vector)
ubiquitin conjugating enzyme E2 I
Gene Symbol
UBE2I
HGNC ID
HGNC:12485
ORF Size (aa)
477
RefSeq ID
NM_003345
RefSeq Synonyms
NM_194261, NM_194260, NM_194259, NM_003345, BC004429, BC000427,
Species
human
UniGene ID
Hs.302903

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.