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human UBE2E3 shRNA silencing Adenovirus

Name 
Ad-h-UBE2E3-shRNA
Cat No 
shADV-227005
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human UBE2E3.

shADV-227005
Ad-h-UBE2E3-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human UBE2E3 (ubiquitin conjugating enzyme E2 E3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
UBCH9; UbcM2
Description (Vector)
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene ID
10477
Gene Name (Vector)
ubiquitin conjugating enzyme E2 E3
Gene Symbol
UBE2E3
HGNC ID
HGNC:12479
ORF Size (aa)
624
RefSeq ID
NM_006357
RefSeq Synonyms
NM_182678, NM_006357, NM_001278555, NM_001278554, BC092407, BC003554,
Species
human
UniGene ID
Hs.470804

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.