human TOPBP1 shRNA silencing Adenovirus

Name 
Ad-h-TOPBP1-shRNA
Cat No 
shADV-226208
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human TOPBP1.

shADV-226208
Ad-h-TOPBP1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human TOPBP1 (topoisomerase (DNA) II binding protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1; Dpb11; TOP2BP1; TopBP1; topoisomerase (DNA) II binding protein 1
Description (eCommerce)
Topoisomerases regulate topological states of DNA. DNA topoisomerase II-binding protein 1 (TopBP1) is a protein that interacts with the C-terminal region of topoisomerase II beta (Topo II beta). TopBP1 function has been found to be important to various aspects of cellular growth control such as DNA replication, the DNA damage checkpoint, and cell survival. TopBP1 interacts with several proteins through its BRCA1 C-terminal (BRCT) motifs. TopBP1 interacts and inhibits E2F1 transcriptional activity through an Rb-independent mechanism that involves recruitment of the SWI/SNF chromatin-remodeling complex proteins Brg1 and Brm.
Description (Vector)
TOPBP1 encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
Gene ID
11073
Gene Name (eCommerce)
DNA topoisomerase II binding protein 1
Gene Name (Vector)
topoisomerase (DNA) II binding protein 1
Gene Symbol
TOPBP1
HGNC ID
HGNC:17008
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
4308
ORF Size (bp)
4308 bp
Protein Name (eCommerce)
DNA topoisomerase 2-binding protein 1
RefSeq ID
NM_007027
RefSeq Synonyms
NM_007027, BC151237, BC126209,
Research Areas
Cell Cycle,Cytoskeleton,DNA Damage/Repair,Fanconi anemia,Homologous Recombination,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
Topoisomerases regulate topological states of DNA. DNA topoisomerase II-binding protein 1 (TopBP1) is a protein that interacts with the C-terminal region of topoisomerase II beta (Topo II beta). TopBP1 function has been found to be important to various aspects of cellular growth control such as DNA replication, the DNA damage checkpoint, and cell survival. TopBP1 interacts with several proteins through its BRCA1 C-terminal (BRCT) motifs. TopBP1 interacts and inhibits E2F1 transcriptional activity through an Rb-independent mechanism that involves recruitment of the SWI/SNF chromatin-remodeling complex proteins Brg1 and Brm.
UniGene ID
Hs.53454
UniProt ID (eCommerce)
Q92547

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.