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human SURF4 shRNA silencing Adenovirus

Name 
Ad-h-SURF4-shRNA
Cat No 
shADV-224759
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SURF4.

shADV-224759
Ad-h-SURF4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SURF4 (surfeit 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ERV29
Description (Vector)
SURF4 is located in the surfeit gene cluster, which is comprised of very tightly linked housekeeping genes that do not share sequence similarity. The encoded protein is a conserved integral membrane protein containing multiple putative transmembrane regions. In eukaryotic cells, protein transport between the endoplasmic reticulum and Golgi compartments is mediated in part by non-clathrin-coated vesicular coat proteins (COPs). The specific function of this protein has not been determined but its yeast homolog is directly required for packaging glycosylated pro-alpha-factor into COPII vesicles. SURF4 uses multiple polyadenylation sites, resulting in transcript length variation. The existence of alternatively spliced transcript variants has been suggested, but their validity has not been determined.
Gene ID
6836
Gene Name (Vector)
surfeit 4
Gene Symbol
SURF4
HGNC ID
HGNC:11476
ORF Size (aa)
810
RefSeq ID
NM_033161
RefSeq Synonyms
NM_033161, NM_001280793, NM_001280792, NM_001280791, NM_001280790, NM_001280789, NM_001280788, BC111023, BC018741,
Species
human
UniGene ID
Hs.512465

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.