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human SMG7 shRNA silencing Adenovirus

Name 
Ad-h-SMG7-shRNA
Cat No 
shADV-223755
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SMG7.

shADV-223755
Ad-h-SMG7-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SMG7 (SMG7, nonsense mediated mRNA decay factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
breast cancer-associated antigen SGA-56M; C1orf16; EST1 telomerase component homolog C; EST1C; EST1-like protein C; ever shorter telomeres 1C; hSMG-7; protein SMG7; SGA56M; SMG-7 homolog; smg-7 homolog, nonsense mediated mRNA decay factor
Description (eCommerce)
SMG7 plays a role in the dephosphorylation of UPF1, an RNA-dependent ATPase and helicase critical to nonsense-mediated mRNA decay (NMD). It is a homolog of the C. elegans NMD factor Smg7 (suppressor with morphogenetic effect on genitalia protein 7). SMG7 functions as an adaptor to target protein phosphatase 2A to UPF1 for dephosphorylation of UPF1.
Description (Vector)
SMG7 encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID
9887
Gene Name (eCommerce)
SMG7, nonsense mediated mRNA decay factor
Gene Name (Vector)
SMG7, nonsense mediated mRNA decay factor
Gene Symbol
SMG7
HGNC ID
HGNC:16792
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3537
ORF Size (bp)
3537 bp
Protein Name (eCommerce)
Protein SMG7
RefSeq ID
NM_173156
RefSeq Synonyms
NM_201569, NM_201568, NM_173156, NM_001331007, NM_001174061, BC036381,
Research Areas
Cytoskeleton,Gene Expression,Kinase/Phosphatase,RNA Processing
Research Areas (Faceted)
cell_biology,genetics,signal_transduction,transcription_translation
Species
human
Target Sentence
SMG7 plays a role in the dephosphorylation of UPF1, an RNA-dependent ATPase and helicase critical to nonsense-mediated mRNA decay (NMD). It is a homolog of the C. elegans NMD factor Smg7 (suppressor with morphogenetic effect on genitalia protein 7). SMG7 functions as an adaptor to target protein phosphatase 2A to UPF1 for dephosphorylation of UPF1.
UniGene ID
Hs.591463
UniProt ID (eCommerce)
Q92540

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.