human PPP4R3A shRNA silencing Adenovirus

Name 
Ad-h-PPP4R3A-shRNA
Cat No 
shADV-223747
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PPP4R3A.

shADV-223747
Ad-h-PPP4R3A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PPP4R3A (SMEK homolog 1, suppressor of mek1 (Dictyostelium)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
FLFL1; KIAA2010; MSTP033; PP4R3; PP4R3A; serine/threonine-protein phosphatase 4 regulatory subunit 3A; SMEK homolog 1; SMEK homolog 1, suppressor of mek1; SMEK1; smk1; smk-1
Description (eCommerce)
PPP4R3 is a binding partner of the PPP4C catalytic subunit of the PP4 serine/threonine protein phosphatase. PPP4C is structurally related to PP2AC but does not associate with the same regulatory subunits. PPP4C associates with either the PPP4R1 or PPP4R2 regulatory subunit. In addition to PPP4R1 and PPP4R2, PPP4R3 has also been identified as a regulator of PPP4C function. PP4R3 functions with PP4C in the control of recovery from DNA damage. PP4R3 may function by targeting PPP4 to sites of DNA damage via an interaction with Rad53 (CHK2). PP4R3 is the human homolog of the Dictylostelium SMEK (suppressor of MEK). SMEK has been shown to be involved in cell polarization and chemotaxis via an involvement in the MEK1/ERK1 signaling pathway. SMEK also functions by controlling PPP4C localization to nucleus.
Gene ID
55671
Gene Name (eCommerce)
protein phosphatase 4 regulatory subunit 3A
Gene Name (Vector)
SMEK homolog 1, suppressor of mek1 (Dictyostelium)
Gene Symbol
PPP4R3A
HGNC ID
HGNC:20219
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2502
ORF Size (bp)
2502 bp
Protein Name (eCommerce)
Serine/threonine-protein phosphatase 4 regulatory subunit 3A
RefSeq ID
NM_032560
RefSeq Synonyms
NM_032560, NM_001284281, NM_001284280, BC072409, BC038932, BC028088,
Research Areas
Cancer,Cell Biology,Cytoskeleton,Signal Transduction
Research Areas (Faceted)
cancer,signal_transduction,cell_biology
Species
human
Target Sentence
PPP4R3 is a binding partner of the PPP4C catalytic subunit of the PP4 serine/threonine protein phosphatase. PPP4C is structurally related to PP2AC but does not associate with the same regulatory subunits. PPP4C associates with either the PPP4R1 or PPP4R2 regulatory subunit. In addition to PPP4R1 and PPP4R2, PPP4R3 has also been identified as a regulator of PPP4C function. PP4R3 functions with PP4C in the control of recovery from DNA damage. PP4R3 may function by targeting PPP4 to sites of DNA damage via an interaction with Rad53 (CHK2). PP4R3 is the human homolog of the Dictylostelium SMEK (suppressor of MEK). SMEK has been shown to be involved in cell polarization and chemotaxis via an involvement in the MEK1/ERK1 signaling pathway. SMEK also functions by controlling PPP4C localization to nucleus.
UniGene ID
Hs.533887
UniProt ID (eCommerce)
Q6IN85

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.