human SILV shRNA silencing Adenovirus

Name 
Ad-h-SILV-shRNA
Cat No 
shADV-223002
Availability
7-8 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PMEL.

shADV-223002
Ad-h-SILV-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PMEL (premelanosome protein). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Validated
No
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV
Description (Vector)
PMEL encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
Gene ID
6490
Gene Name (Vector)
premelanosome protein
Gene Symbol
PMEL
HGNC ID
HGNC:10880
ORF Size (aa)
1986
ORF Size (bp)
1986 bp
RefSeq ID
NM_006928
RefSeq Synonyms
NM_006928, NM_001320122, NM_001320121, NM_001200054, NM_001200053, BC001414,
Species
human
UniGene ID
Hs.95972

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.