human SF3B4 shRNA silencing Adenovirus

Name 
Ad-h-SF3B4-shRNA
Cat No 
shADV-222763
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SF3B4.

shADV-222763
Ad-h-SF3B4-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SF3B4 (splicing factor 3b subunit 4). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AFD1; Hsh49; pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49; SF3b49; SF3b50; spliceosomal protein; spliceosome-associated protein (U2 snRNP); spliceosome-associated protein 49; splicing factor 3B subunit 4; splicing factor 3b, subunit 4, 49kD; splicing factor 3b, subunit 4, 49kDa
Description (eCommerce)
Splicing factor 3b, subunit 4, 49kDa (SF3B4) is one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B [taken from NCBI Entrez Gene (Gene ID: 10262)].
Description (Vector)
SF3B4 encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.
Gene ID
10262
Gene Name (eCommerce)
splicing factor 3b subunit 4
Gene Name (Vector)
splicing factor 3b subunit 4
Gene Symbol
SF3B4
HGNC ID
HGNC:10771
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1275
ORF Size (bp)
1275 bp
Protein Name (eCommerce)
Splicing factor 3B subunit 4
RefSeq ID
NM_005850
RefSeq Synonyms
NM_005850, BC090883, BC013886, BC004273,
Research Areas
Gene Expression,mRNA Processing,mRNA Splicing,RNA Binding,RNA Processing,Spliceosome
Research Areas (Faceted)
genetics,transcription_translation,cell_biology
Species
human
Target Sentence
Splicing factor 3b, subunit 4, 49kDa (SF3B4) is one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B [taken from NCBI Entrez Gene (Gene ID: 10262)].
UniGene ID
Hs.516160
UniProt ID (eCommerce)
Q15427

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.