human SF3B14 shRNA silencing Adenovirus

Name 
Ad-h-SF3B14-shRNA
Cat No 
shADV-222761
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human SF3B6.

shADV-222761
Ad-h-SF3B14-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SF3B6 (splicing factor 3b subunit 6). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a
Description (Vector)
SF3B14 encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
Gene ID
51639
Gene Name (Vector)
splicing factor 3b subunit 6
Gene Symbol
SF3B6
HGNC ID
HGNC:30096
ORF Size (aa)
378
RefSeq ID
NM_016047
RefSeq Synonyms
NM_016047, BC015463,
Species
human
UniGene ID
Hs.177861

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.