human SEMA5B shRNA silencing Adenovirus

Name
Ad-h-SEMA5B-shRNA

Cat No
shADV-222561

Availability
7-8 weeks

This is an Adenovirus expressing shRNA for silencing of Human SEMA5B.

shADV-222561

Ad-h-SEMA5B-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SEMA5B (semaphorin 5B). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Validated

Storage Buffer

DMEM, 2% BSA, 2.5% Glycerol

Viral Backbone

Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names

SEMAG; SemG

Description (Vector)

SEMA5B encodes a member of the semaphorin protein family which regulates axon growth during development of the nervous system. The encoded protein has a characteristic Sema domain near the N-terminus, through which semaphorins bind to plexin, and five thrombospondin type 1 repeats in the C-terminal region of the protein. The protein product may be cleaved and exist as a secreted molecule (PMID: 19463192). Multiple transcript variants encoding different isoforms have been found for this gene.

Gene ID

54437

Gene Name (Vector)

semaphorin 5B

Gene Symbol

SEMA5B

HGNC ID

HGNC:10737

ORF Size (aa)

3456

ORF Size (bp)

3456 bp

RefSeq ID

NM_001256346

RefSeq Synonyms

NM_001256348, NM_001256347, NM_001256346, NM_001031702, BC077726,

Species

human

UniGene ID

Hs.210870

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.