human SEMA4G shRNA silencing Adenovirus

Name 
Ad-h-SEMA4G-shRNA
Cat No 
shADV-222559
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human SEMA4G.

shADV-222559
Ad-h-SEMA4G-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SEMA4G (semaphorin 4G). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Description (Vector)
Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
57715
Gene Name (Vector)
semaphorin 4G
Gene Symbol
SEMA4G
HGNC ID
HGNC:10735
ORF Size (aa)
2109
ORF Size (bp)
2109 bp
RefSeq ID
NM_017893
RefSeq Synonyms
NM_017893, NM_001203244, BC128579, BC110382, BC051030, BC020960,
Species
human
UniGene ID
Hs.591923

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.