human SCNN1A shRNA silencing Adenovirus

Name 
Ad-h-SCNN1A-shRNA
Cat No 
shADV-222377
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human SCNN1A.

shADV-222377
Ad-h-SCNN1A-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human SCNN1A (sodium channel epithelial 1 alpha subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
BESC2; ENaCa; ENaCalpha; SCNEA; SCNN1
Description (Vector)
Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Gene ID
6337
Gene Name (Vector)
sodium channel epithelial 1 alpha subunit
Gene Symbol
SCNN1A
HGNC ID
HGNC:10599
ORF Size (aa)
2010
ORF Size (bp)
2010 bp
RefSeq ID
NM_001038
RefSeq Synonyms
NM_001159576, NM_001159575, NM_001038, BC062613,
Species
human
UniGene ID
Hs.591047

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.