human REV1 shRNA silencing Adenovirus

Name 
Ad-h-REV1-shRNA
Cat No 
shADV-221024
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human REV1.

shADV-221024
Ad-h-REV1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human REV1 (REV1, DNA directed polymerase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
AIBP80; REV1L
Description (Vector)
REV1 encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found.
Gene ID
51455
Gene Name (Vector)
REV1, DNA directed polymerase
Gene Symbol
REV1
HGNC ID
HGNC:14060
ORF Size (aa)
3345
ORF Size (bp)
3345 bp
RefSeq ID
NM_016316
RefSeq Synonyms
NM_016316, NM_001321460, NM_001321459, NM_001321458, NM_001321455, NM_001321454, NM_001037872, BC130411, BC037734,
Species
human
UniGene ID
Hs.443077

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.