human PPT1 shRNA silencing Adenovirus

Name 
Ad-h-PPT1-shRNA
Cat No 
shADV-219694
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human PPT1.

shADV-219694
Ad-h-PPT1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PPT1 (palmitoyl-protein thioesterase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
CLN1; INCL; PPT
Description (Vector)
PPT1 is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
5538
Gene Name (Vector)
palmitoyl-protein thioesterase 1
Gene Symbol
PPT1
HGNC ID
HGNC:9325
ORF Size (aa)
921
RefSeq ID
NM_000310
RefSeq Synonyms
NM_001142604, NM_000310, BC008426,
Species
human
UniGene ID
Hs.3873

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.