human PPP1CB shRNA silencing Adenovirus

Name 
Ad-h-PPP1CB-shRNA
Cat No 
shADV-219599
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PPP1CB.

shADV-219599
Ad-h-PPP1CB-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PPP1CB (protein phosphatase 1 catalytic subunit beta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
epididymis secretory sperm binding protein Li 80p; HEL-S-80p; NSLH2; PP1B; PP-1B; PP1beta; PPP1CD; protein phosphatase 1, catalytic subunit, beta isoform; protein phosphatase 1, catalytic subunit, beta isozyme; protein phosphatase 1, catalytic subunit, delta isoform; protein phosphatase 1-beta; protein phosphatase 1-delta; serine/threonine-protein phosphatase PP1-beta catalytic subunit
Description (eCommerce)
Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine phosphatase that is involved in a multitude of cellular functions. PP1 is a holoenzyme that consists of the PP1 catalytic subunit (PP1c) that associates with and is regulated by over 50 regulatory subunits. There are several gene and alternative splice products of the catalytic subunit PP1c. PPP1CB encodes the PP1 beta catalytic subunit. In addition to PP1 beta, there are two splice variants of PP1 alpha (PPP1CA), and PP1 gamma (PPP1CC). The mutually exclusive interaction between the various catalytic and regulatory subunits allows the PP1 enzyme to be involved in a variety of functions which include cell division, glycogen metabolism, muscle contractility, and protein synthesis. serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, MGC3672.
Description (Vector)
PPP1CB is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene ID
5500
Gene Name (eCommerce)
protein phosphatase 1 catalytic subunit beta
Gene Name (Vector)
protein phosphatase 1 catalytic subunit beta
Gene Symbol
PPP1CB
HGNC ID
HGNC:9282
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
984
ORF Size (bp)
984 bp
Protein Name (eCommerce)
Serine/threonine-protein phosphatase PP1-beta catalytic subunit
RefSeq ID
NM_002709
RefSeq Synonyms
NM_206876, NM_002709, BC012045, BC002697,
Research Areas
Cancer,Cardiovascular,Cell Cycle,Cell Division,Cytoskeleton,Hippo Signaling,Host-Virus Interactions,Insulin Signaling,Kinase/Phosphatase,Meiosis,Metabolism/Metabolic Process,Neurobiology,Phosphatase,PLK1 Signaling,Signal Transduction,TGF-beta Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,immunology,infectious_disease,metabolism,neurobiology,signal_transduction
Species
human
Target Sentence
Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine phosphatase that is involved in a multitude of cellular functions. PP1 is a holoenzyme that consists of the PP1 catalytic subunit (PP1c) that associates with and is regulated by over 50 regulatory subunits. There are several gene and alternative splice products of the catalytic subunit PP1c. PPP1CB encodes the PP1 beta catalytic subunit. In addition to PP1 beta, there are two splice variants of PP1 alpha (PPP1CA), and PP1 gamma (PPP1CC). The mutually exclusive interaction between the various catalytic and regulatory subunits allows the PP1 enzyme to be involved in a variety of functions which include cell division, glycogen metabolism, muscle contractility, and protein synthesis. serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, MGC3672.
UniGene ID
Hs.702907
UniProt ID (eCommerce)
P62140

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.