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human PPAT shRNA silencing Adenovirus

Name 
Ad-h-PPAT-shRNA
Cat No 
shADV-219516
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human PPAT.

shADV-219516
Ad-h-PPAT-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PPAT (phosphoribosyl pyrophosphate amidotransferase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
ATASE; GPAT; PRAT
Description (Vector)
PPAT is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.
Gene ID
5471
Gene Name (Vector)
phosphoribosyl pyrophosphate amidotransferase
Gene Symbol
PPAT
HGNC ID
HGNC:9238
ORF Size (aa)
1554
ORF Size (bp)
1554 bp
RefSeq ID
NM_002703
RefSeq Synonyms
NM_002703, BC004200,
Species
human
UniGene ID
Hs.708542

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.