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human POLR2I shRNA silencing Adenovirus

Name 
Ad-h-POLR2I-shRNA
Cat No 
shADV-219400
Availability
4-5 weeks

This is an Adenovirus expressing shRNA for silencing of Human POLR2I.

shADV-219400
Ad-h-POLR2I-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human POLR2I (RNA polymerase II subunit I). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
hRPB14.5; RPB9
Description (Vector)
POLR2I encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity.
Gene ID
5438
Gene Name (Vector)
RNA polymerase II subunit I
Gene Symbol
POLR2I
HGNC ID
HGNC:9196
ORF Size (aa)
378
RefSeq ID
NM_006233
RefSeq Synonyms
NM_006233, BC067794, BC017112,
Species
human
UniGene ID
Hs.47062

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.