human PLOD1 shRNA silencing Adenovirus

Name 
Ad-h-PLOD1-shRNA
Cat No 
shADV-219218
Availability
4-5 weeks
Categories
Adenovirus, shRNA-Silencing

This is an Adenovirus expressing shRNA for silencing of Human PLOD1.

shADV-219218
Ad-h-PLOD1-shRNA

Ready-to-use Adenovirus expressing shRNA for silencing of Human PLOD1 (procollagen-lysine,2-oxoglutarate 5-dioxygenase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
shRNA Validated
Yes
Storage Buffer
DMEM, 2% BSA, 2.5% Glycerol
Viral Backbone
Human Adenovirus Type5 (dE1/E3)

Gene Reference Data

Alternate Names
EDS6; LH; LH1; LLH; PLOD
Description (Vector)
Lysyl hydroxylase is a membrane-bound homodimeric protein localized to the cisternae of the endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VI have deficiencies in lysyl hydroxylase activity.
Gene ID
5351
Gene Name (Vector)
procollagen-lysine,2-oxoglutarate 5-dioxygenase 1
Gene Symbol
PLOD1
HGNC ID
HGNC:9081
ORF Size (aa)
2184
ORF Size (bp)
2184 bp
RefSeq ID
NM_000302
RefSeq Synonyms
NM_001316320, NM_000302, BC016657,
Species
human
UniGene ID
Hs.75093

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.